Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- NBP2-61784 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Phospholipase D2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references HIF1α/PLD2 axis linked to glycolysis induces T-cell immunity in oral lichen planus.
Wang F, Zhang J, Zhou G
Biochimica et biophysica acta. General subjects 2020 Jul;1864(7):129602
Biochimica et biophysica acta. General subjects 2020 Jul;1864(7):129602
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Phospholipase D2 Antibody (7E4D9) [NBP2-61784] - Analysis using PLD2 mAb against HEK293 (1) and PLD2 (AA: 834-933)-hIgGFc transfected HEK293 (2) cell lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Phospholipase D2 Antibody (7E4D9) [NBP2-61784] - Analysis using PLD2 mAb against human PLD2 (AA: 834-933) recombinant protein. (Expected MW is 37.4 kDa)
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Phospholipase D2 Antibody (7E4D9) [NBP2-61784] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Phospholipase D2 Antibody (7E4D9) [NBP2-61784] - Analysis of paraffin-embedded renal tissues using PLD2 mouse mAb with DAB staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Phospholipase D2 Antibody (7E4D9) [NBP2-61784] - Analysis of paraffin-embedded esophageal cancer tissues using PLD2 mouse mAb with DAB staining.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Phospholipase D2 Antibody (7E4D9) [NBP2-61784] - Analysis of Hela cells using PLD2 mouse mAb (green) and negative control (red).