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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- ELISA [1]
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- Product number
- H00005338-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00005338-M01, RRID:AB_2237053
- Product name
- Mouse Monoclonal Phospholipase D2 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. PLD2 - phospholipase D2
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Choline Is an Intracellular Messenger Linking Extracellular Stimuli to IP3-Evoked Ca2+ Signals through Sigma-1 Receptors.
Inhibition of PLD1 activity causes ER stress via regulation of COPII vesicle formation.
Mechanisms for the activity of heterocyclic cyclohexanone curcumin derivatives in estrogen receptor negative human breast cancer cell lines.
Brailoiu E, Chakraborty S, Brailoiu GC, Zhao P, Barr JL, Ilies MA, Unterwald EM, Abood ME, Taylor CW
Cell reports 2019 Jan 8;26(2):330-337.e4
Cell reports 2019 Jan 8;26(2):330-337.e4
Inhibition of PLD1 activity causes ER stress via regulation of COPII vesicle formation.
Nakagawa H, Hazama K, Ishida K, Komori M, Nishimura K, Matsuo S
Biochemical and biophysical research communications 2017 Aug 26;490(3):895-900
Biochemical and biophysical research communications 2017 Aug 26;490(3):895-900
Mechanisms for the activity of heterocyclic cyclohexanone curcumin derivatives in estrogen receptor negative human breast cancer cell lines.
Somers-Edgar TJ, Taurin S, Larsen L, Chandramouli A, Nelson MA, Rosengren RJ
Investigational new drugs 2011 Feb;29(1):87-97
Investigational new drugs 2011 Feb;29(1):87-97
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Phospholipase D2 Antibody (1C5) [H00005338-M01] - Analysis of PLD2 expression in transfected 293T cell line by PLD2 monoclonal antibody (M01), clone 1C5.Lane 1: PLD2 transfected lysate (Predicted MW: 105.9 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Phospholipase D2 Antibody (1C5) [H00005338-M01] - PLD2 monoclonal antibody (M01), clone 1C5. Analysis of PLD2 expression in rat brain.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Phospholipase D2 Antibody (1C5) [H00005338-M01] - Detection limit for recombinant GST tagged PLD2 is approximately 0.03ng/ml as a capture antibody.
