OAAB03724

antibody from Aviva Systems Biology

Targeting: SPCS3 FLJ22649, PRO3567, SPC22/23, SPC3, YLR066W

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

OAAB03724

Provider

Aviva Systems Biology

Product name

SPCS3 antibody - C-terminal region

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

Vial size

400ul

Storage

Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Aviva Systems Biology (provider)

Main image

Experimental details

Western blot analysis of SPCS3 antibody (C-term) in mouse liver tissue lysates (35ug/lane). SPCS3 (arrow) was detected using the purified Pab.

Sample type

mouse liver tissue lysates

Primary Ab dilution

1.0 µg/mL

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Aviva Systems Biology (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with SPCS3 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Sample type

human hepatocarcinoma

Primary Ab dilution

1.0 µg/mL

Protocol

Protocol

Flow cytometry

Supportive validation

Submitted by

Aviva Systems Biology (provider)

Main image

Experimental details

SPCS3 Antibody (C-term) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Sample type

HepG2 cells

Primary Ab dilution

1.0 µg/mL

Protocol

Protocol