MAB9219-100

antibody from R&D Systems

Targeting: MESP1 bHLHc5, MGC10676

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB9219-100

Provider

R&D Systems

Product name

Human MESP1 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from cell culture supernatant. Detects human MESP1 in direct ELISAs.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

Q9BRJ9


Isotype

IgG

Antibody clone number

2030B

Vial size

100 ug

Concentration

1.0 mg/ml

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human MESP1 by Western Blot. Western blot shows lysates of Mouse ES cells transfected with human MESP1 untreated (-) or treated (+) with 100 ng/mL Doxycycline overnight. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human MESP1 Monoclonal Antibody (Catalog # MAB9219) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for MESP1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3. Cells provided courtesy of Michael Kyba's lab, University of Minnesota.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human MESP1 by Western Blot. Western blot shows lysates of H1 human embryonic stem cells untreated (-) or treated (+) with 100 ng/mL Doxycycline overnight. PVDF membrane was probed with Rabbit Anti-Human MESP1 Monoclonal Antibody (Catalog # MAB9219) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for MESP1 at approximately 30 kDa (as indicated). Image provided courtesy of Michael Kyba's lab, University of Minnesota.

Immunocytochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Human MESP1 in H1 Human Embryonic Stem Cells. MESP1 was detected in fixed H1 human embryonic stem cells untreated (lower panel) or treated (upper panel) with Doxycycline using Rabbit Anti-Human MESP1 Monoclonal Antibody (Catalog # MAB9219). Cells were stained using anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Image provided courtesy of Michael Kyba's lab, University of Minnesota.

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of MESP1 in Mouse ES cells transfected with human MESP1 by Flow Cytometry. Mouse ES cells transfected with human MESP1 were stained with Rabbit Anti-Human MESP1 Monoclonal Antibody (Catalog # MAB9219, filled histogram) or isotype control antibody (Catalog # MAB1050, open histogram), followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.