PA5-47922

antibody from Invitrogen Antibodies

Targeting: BSCL2 GNG3LG, seipin, SPG17

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-47922

Provider

Invitrogen Antibodies

Product name

Seipin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Reconstitute in sterile PBS to a final concentration of 0.2 mg/mL.

Reactivity

Human

Host

Sheep

Isotype

IgG

Vial size

100 µg

Concentration

0.2 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

PyMINEr Finds Gene and Autocrine-Paracrine Networks from Human Islet scRNA-Seq.

Tyler SR, Rotti PG, Sun X, Yi Y, Xie W, Winter MC, Flamme-Wiese MJ, Tucker BA, Mullins RF, Norris AW, Engelhardt JF
Cell reports 2019 Feb 12;26(7):1951-1964.e8

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis from lysates of IMR-32 human neuroblastoma cell line and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-human Seipin/BSCL2 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47922) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. Specific bands were detected for Seipin/BSCL2 at approximately 70 and 40 kDa (as indicated). This experiment was conducted under reducing conditions.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Seipin in IMR‚32 human neuroblastoma cell line and HEK293 human embryonic kidney cell line. Samples were incubated in Seipin polyclonal antibody (Product # PA5-47922) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Sheep IgG secondary antibody. Specific bands were detected for Seipin/BSCL2 at approximately 70 and 40 kDa (as indicated). This experiment was conducted under reducing conditions.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of Seipin in immersion fixed human mesenchymal stem cells differentiated into adipocytes. Samples were incubated in Seipin polyclonal antibody (Product # PA5-47922) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 5. Cell Type-Specific Enrichment of T2D-Associated Genes (A) A subset of the larger networks shown in Figure 2E focused on T2D-associated genes. The nature of the associations that were previously published is indicated by color ( Morris et al., 2012 ). Gene associations newly discovered through the cited meta-analysis are denoted as ""newly associated locus."" (B-I) The Z score enrichment of T2D-associated genes by cell type (from our dataset) are displayed over the two largest connected components of the T2D gene subset of the transcriptional network built by PyMINEr. As indicated, panels correspond to beta (B), epsilon (C), pancreatic polypeptide cells (D), ductal (E), stromal (F), delta (G), acinar (H), and alpha cells (I). Highly enriched genes are shown in red, whereas genes whose expression is low in the given cell type are shown in blue. If a gene passed the threshold for significant enrichment for the given cell type, then it is highlighted with a cyan ring. Table S2A lists cell type annotations for all T2D-associated genes, including those not shown here. (J) Immunofluorescence staining of a human pancreas section for glucagon (GCG; alpha cells, red), BSCL2 (green), and insulin (INS; beta cells, white) and counterstaining with Hoechst dye (nuclei, blue) (n = 5). Although many alpha cells were positive for BSCL2, we also observed expression in other islet cells (examples noted with arrows). (K) Representative immunofluorescence staining of a human pancreas sec