HPA014492

antibody from Atlas Antibodies

Targeting: PDE3A CGI-PDE

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA014492

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA014492, RRID:AB_1855120

Product name

Anti-PDE3A

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

SPQILTPPVICSSCGRPYSQGNPADEPLERSGVAT
RTPSRTDDTAQVTSDYETNNNSDSSDIVQNEDETE
CLREPLRKASACSTYAPETMMFLDKPILAPEPLVM
DNLDSIMEQLNT


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Western blot analysis in human cell lines HeLa and HEK293 using Anti-PDE3A antibody. Corresponding PDE3A RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.

Orthogonal method

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in human cell line HEL.

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line HeLa shows localization to cytosol.

Sample type

HUMAN

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.

Sample type

HUMAN