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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- H00001635-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00001635-M01, RRID:AB_538190
- Product name
- Mouse Monoclonal Deoxycytidylate deaminase Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. DCTD - dCMP deaminase
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Deoxycytidylate deaminase Antibody (4B9) [H00001635-M01] - Analysis of DCTD over-expressed 293 cell line, cotransfected with DCTD Validated Chimera RNAi ( Cat # H00001635-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with DCTD monoclonal antibody (M01), clone 4B9 (Cat # H00001635-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Deoxycytidylate deaminase Antibody (4B9) [H00001635-M01] - Analysis of DCTD expression in transfected 293T cell line by DCTD monoclonal antibody (M01), clone 4B9.Lane 1: DCTD transfected lysate(20.016 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: Deoxycytidylate deaminase Antibody (4B9) [H00001635-M01] - Detection limit for recombinant GST tagged DCTD is 0.03 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Deoxycytidylate deaminase Antibody (4B9) [H00001635-M01] - Analysis of monoclonal antibody to DCTD on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.
