PA5-40738

antibody from Invitrogen Antibodies

Targeting: ZNF217 ZABC1

Antibody data

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Validation data

Product number: PA5-40738 - Provider product page
Provider: Invitrogen Antibodies
Product name: ZNF217 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: Peptide sequence: SQTFTHSEDL NKHVLMQHRP TLCEPAVLRV EAEYLSPLDK SQVRTEPPKE
Concentration: 0.5 mg/mL

ZNF217 protein structure - PA5-40738 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 4 ZNF217 is a target of miR-107. (A) The potential binding sites of miR-107 and ZNF-217. (B) Luciferase activity was measured in PC12 cells after co-transfection of ZNF217-WT or ZNF217-MUT and miR-107 mimic or miR-NC. (C) The enrichment of ZNF217 was detected in PC12 cells transfected with miR-107 mimic or miR-NC after RIP assay. (D) The protein level of ZNF217 was measured in PC12 cells transfected with miR-NC, miR-107 mimic, anti-miR-107 or anti-miR-NC by Western blot. ** P < 0.01, *** P < 0.001, ns: no significance, P > 0.05.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 5 miR-107 suppresses Abeta 25-35 -induced injury in PC12 cells by regulating ZNF217. PC12 cells were transfected with miR-NC, miR-107 mimic, miR-107 mimic and ZNF217 or pcDNA for 24 h and then treated with 1 muM berberine and 10 muM Abeta for 24 h. The ZNF217 protein level (A), expressions of IL-1beta, IL-6 and TNF-alpha in cell medium (B-D), cell apoptosis (E) and p-Tau level (F) were detected by Western blot, ELISA or flow cytometry assays, respectively. * P < 0.05, ** P < 0.01, *** P < 0.001.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Berberine decreases ZNF217 protein expression by regulating miR-107 in Abeta 25-35 -treated PC12 cells. PC12 cells were transfected with miR-NC, miR-107 mimic, anti-miR-NC or anti-miR-107 for 24 h and treated with 1 muM berberine and 10 muM Abeta for 24 h. (A and B) The abundance of ZNF217 protein was measured in treated PC12 cells by Western blot. * P < 0.05, *** P < 0.001.