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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- PA5-60797 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SPDL1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: YEPEETVEVP VLKKRREVLP VDITTAKDAC VNNSALGGEV YRLPPQKEET QSCPNSLEDN NLQLEKSVSI HTPVVSLSPH KNLPVDMQLK KEKKCVKLIG VPADA
- Concentration
- 0.2 mg/mL
Submitted references SPDL1 Is an Independent Predictor of Patient Outcome in Colorectal Cancer.
Klimaszewska-Wiśniewska A, Buchholz K, Durślewicz J, Villodre ES, Gagat M, Grzanka D
International journal of molecular sciences 2022 Feb 5;23(3)
International journal of molecular sciences 2022 Feb 5;23(3)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SPDL1 in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using a SPDL1 Polyclonal Antibody (Product # PA5-60797). Remaining relative intensity is presented.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SPDL1 in human testis and liver tissues using SPDL1 Polyclonal Antibody (Product # PA5-60797). Corresponding SPDL1 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SPDL1 in human testis using SPDL1 Polyclonal Antibody (Product # PA5-60797) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SPDL1 in human liver using SPDL1 Polyclonal Antibody (Product # PA5-60797) shows low expression as expected.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 ( A ) Immunohistochemical expression of SPDL1 in colorectal cancer compared to non-cancerous adjacent tissues. The error bars present the range from minimum to maximum values of data. Asterisk (*) indicates statistically significant difference ( p < 0.05). ( B ) The optimal cut-off value established for SPDL1 based on IRS scores and Evaluate Cutpoints software.
