24689-1-AP

antibody from Invitrogen Antibodies

Targeting: SPDL1 CCDC99, FLJ20364, hSpindly

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

24689-1-AP

Provider

Invitrogen Antibodies

Product name

CCDC99 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: AEVEDRRAA MERQLISMKV KYQSLKKQNV FNREQMQRMK LQIATLLQMK GSQTEFEQQE RLLAMLEQKN GEIKHLLGEI RNLEKFKNLY DSMESKPSVD SGTLEDNTYY TDLLQMKLDN LNKEIESTKG ELSIQRMKAL FESQRALDIE RKLFANERCL QLSESENMKL RAKLDELKLK YEPEETVEVP VLKKRREVLP VDITTAKDAC VNNSALGGEV YRLPPQKEET QSCPNSLEDN NLQLEKSVSI HTPVVSLSPH KNLPVDMQLK KEKKCVKLIG VPADAEALSE RSGNTPNSPR LAAESKLQTE VKEGKETSSK LEKETCKKSH PILYVSSKST PETQCPQQ (259-605 aa encoded by BC012568)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.28 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 24689-1-AP (CCDC99 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Various lysates were subjected to SDS PAGE followed by western blot with 24689-1-AP (CCDC99 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 24689-1-AP (CCDC99 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 24689-1-AP ( CCDC99 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human brain slide using 24689-1-AP (CCDC99 Antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis slide using 24689-1-AP (CCDC99 Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis slide using 24689-1-AP (CCDC99 Antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer slide using 24689-1-AP ( CCDC99 Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer slide using 24689-1-AP ( CCDC99 Antibody) at dilution of 1:50 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-CCDC99 (IP:24689-1-AP, 3ug; Detection:24689-1-AP 1:1000) with HEK-293 cells lysate 920ug.