35-4500

antibody from Invitrogen Antibodies

Targeting: CIB1 CIB, KIP, SIP2-28

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

35-4500

Provider

Invitrogen Antibodies

Product name

Calmyrin Monoclonal Antibody (1G8C8)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

1G8C8

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

-20°C

References

Submitted references

Differential membrane proteomics using 18O-labeling to identify biomarkers for cholangiocarcinoma.

Kristiansen TZ, Harsha HC, Grønborg M, Maitra A, Pandey A
Journal of proteome research 2008 Nov;7(11):4670-7

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on tissue extract (30 µg lysate) of Mouse Brain (Lane 1). The blots were probed with Anti-Calmyrin Mouse Monoclonal Antibody (Product # 35-4500, 1-3 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.4 µg/mL, 1:2500 dilution). A 21 kDa band corresponding to Calmyrin was observed in the tissue tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).