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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- MAB8297 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB8297, RRID:AB_10615766
- Product name
- CIB1 monoclonal antibody, clone 5A1F5E12, 5A1H7E12
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant CIB1.
- Antibody clone number
- 5A1F5E12, 5A1H7E12
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references The interaction between calcium- and integrin-binding protein 1 and the alphaIIb integrin cytoplasmic domain involves a novel C-terminal displacement mechanism.
CIB1, a ubiquitously expressed Ca2+-binding protein ligand of the InsP3 receptor Ca2+ release channel.
Structural and biochemical characterization of CIB1 delineates a new family of EF-hand-containing proteins.
Yamniuk AP, Ishida H, Vogel HJ
The Journal of biological chemistry 2006 Sep 8;281(36):26455-64
The Journal of biological chemistry 2006 Sep 8;281(36):26455-64
CIB1, a ubiquitously expressed Ca2+-binding protein ligand of the InsP3 receptor Ca2+ release channel.
White C, Yang J, Monteiro MJ, Foskett JK
The Journal of biological chemistry 2006 Jul 28;281(30):20825-33
The Journal of biological chemistry 2006 Jul 28;281(30):20825-33
Structural and biochemical characterization of CIB1 delineates a new family of EF-hand-containing proteins.
Gentry HR, Singer AU, Betts L, Yang C, Ferrara JD, Sondek J, Parise LV
The Journal of biological chemistry 2005 Mar 4;280(9):8407-15
The Journal of biological chemistry 2005 Mar 4;280(9):8407-15
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using CIB1 monoclonal antibody, clone 5A1F5E12, 5A1H7E12 (Cat # MAB8297) against truncated CIB1 recombinant protein (Lane 1) and A-431 cell lysate (Lane 2).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human thalamus tissue (A) and glioma tissue (B), showing membrane localization using CIB1 monoclonal antibody, clone 5A1F5E12, 5A1H7E12 (Cat # MAB8297) with DAB staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
