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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [9]
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- Product number
- UM800039 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#UM800039, RRID:AB_2629151
- Product name
- DOCK2 mouse monoclonal antibody,clone UMAB142
- Antibody type
- Monoclonal
- Description
- DOCK2 mouse monoclonal antibody,clone UMAB142
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- DOCK2
- Isotype
- IgG
- Antibody clone number
- UMAB142
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of RPMI8226 cell lysate (35ug) by using anti-DOCK2 monoclonal antibody.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of Jurkat cells using anti-DOCK2 mouse monoclonal antibody (UM800039, green, 1:100). Actin filaments were labeled with Alexa Fluor? 594 Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 20?m.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-DOCK2 mouse monoclonal antibody. (UM800039; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-DOCK2 mouse monoclonal antibody. (UM800039; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-DOCK2 mouse monoclonal antibody. (UM800039; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-DOCK2 mouse monoclonal antibody. (UM800039; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human tonsil using anti-DOCK2 mouse monoclonal antibody. (UM800039; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human lung cancer using anti-DOCK2 clone UMAB142 mouse monoclonal antibody at 1:200 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. UM800039 requires HIER with with citrate pH6.0 at 110C for 3 min using pressure chamber/cooker. IHC staining shows tumor cells are negative however infiltrating lymphocytes strongly positive.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human spleen using anti-DOCK2 clone UMAB142 mouse monoclonal antibody at 1:200 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. UM800039 requires HIER with with citrate pH6.0 at 110C for 3 min using pressure chamber/cooker. Show strong cytoplasmic and membrane staining.
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human tonsil using anti-DOCK2 clone UMAB142 mouse monoclonal antibody at 1:200 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. UM800039 requires HIER with with citrate pH6.0 at 110C for 3 min using pressure chamber/cooker. IHC staining shows germinal and nongerminal center with strong stain in the cytoplasm and membrane.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human melanoma using anti-DOCK2 clone UMAB142 mouse monoclonal antibody at 1:200 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. UM800039 requires HIER with with citrate pH6.0 at 110C for 3 min using pressure chamber/cooker. IHC staining shows tumor cells are negative however infiltrating lymphocytes strongly positive.
- Validation comment
- IHC
