Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- 11082-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PPP1CC Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MADLDKLNI DSIIQRLLEV RGSKPGKNVQ LQENEIRGLC LKSREIFLSQ PILLELEAPL KICGDIHGQY YDLLRLFEYG GFPPESNYLF LGDYVDRGKQ SLETICLLLA YKIKYPENFF LLRGNHECAS INRIYGFYDE CKRRYNIKLW KTFTDCFNCL PIAAIVDEKI FCCHGGLSPD LQSMEQIRRI MRPTDVPDQG LLCDLLWSDP DKDVLGWGEN DRGVSFTFGA EVVAKFLHKH DLDLICRAHQ VVEDGYEFFA KRQLVTLFSA PNYCGEFDNA GAMMSVDETL MCSFQILKPA EKKKPNATRP VTPPRGMITK QAKK (1-323 aa encoded by BC014073)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.13 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF7 cells were subjected to SDS PAGE followed by western blot with 11082-1-AP (PPP1CC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 11082-1-AP (PPP1CC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- L02 cells were subjected to SDS PAGE followed by western blot with 11082-1-AP (PPP1CC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates were subjected to SDS PAGE followed by western blot with 11082-1-AP (PPP1CC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293T cells were subjected to SDS PAGE followed by western blot with 11082-1-AP (PPP1CC Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HEK-293 cells using 11082-1-AP (PPP1CC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded tissue slide using 11082-1-AP (PPP1CC antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded tissue slide using 11082-1-AP (PPP1CC antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-PPP1CC (IP:11082-1-AP, 4ug; Detection:11082-1-AP 1:500) with HEK-293 cells lysate 2000ug.