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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Other assay [2]
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- Product number
- PA5-21671 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PP1 gamma Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, Molt-4, Raji, NIH-3T3.
- Concentration
- 0.79 mg/mL
Submitted references Ultrastructural Localization and Molecular Associations of HCV Capsid Protein in Jurkat T Cells.
Cohesin Removal along the Chromosome Arms during the First Meiotic Division Depends on a NEK1-PP1γ-WAPL Axis in the Mouse.
Fernández-Ponce C, Durán-Ruiz MC, Narbona-Sánchez I, Muñoz-Miranda JP, Arbulo-Echevarria MM, Serna-Sanz A, Baumann C, Litrán R, Aguado E, Bloch W, García-Cozar F
Frontiers in microbiology 2017;8:2595
Frontiers in microbiology 2017;8:2595
Cohesin Removal along the Chromosome Arms during the First Meiotic Division Depends on a NEK1-PP1γ-WAPL Axis in the Mouse.
Brieño-Enríquez MA, Moak SL, Toledo M, Filter JJ, Gray S, Barbero JL, Cohen PE, Holloway JK
Cell reports 2016 Oct 18;17(4):977-986
Cell reports 2016 Oct 18;17(4):977-986
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PP1 gamma using 30 µg of A) HepG2 and B) MOLT4 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a PP1 gamma polyclonal antibody (Product # PA5-21671) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PP1 gamma Polyclonal Antibody (Product # PA5-21671). Sample (30 µg of whole cell lysate). Lane A: NIH-3T3 10% SDS PAGE. PP1 gamma Polyclonal Antibody (Product # PA5-21671) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PP1 gamma Polyclonal Antibody (Product # PA5-21671). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PP1 gamma Polyclonal Antibody (Product # PA5-21671) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PP1 gamma in paraformaldehyde-fixed HeLa cells using a PP1 gamma polyclonal antibody (Product # PA5-21671) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PP1 gamma antibody (Product # PA5-21671) at 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SAS xenograft, using PPP1CC (Product # PA5-21671) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 HCV Core protein interacts with PP1gamma and MYPT1 in PBMC blasts. Postnuclear lysates from Human PBMCs stimulated with PHA and cultured during 5 days with IL-2, were subjected to HCV Core protein pull down. Proteins pulled down by HCV Core coated magnetic beads (PBMC Core band) or by uncoated magnetic beads (PBMC beads band) were analyzed by Western blot with anti-human MYPT1 (A) and anti-human PP1gamma (B). Molecular mass, in kDa, is indicated on the side and band quantitation at the bottom for each Western blot.
