HPA019769

antibody from Atlas Antibodies

Targeting: TRIOBP DFNB28, HRIHFB2122, KIAA1662, TAP68, Tara

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA019769

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA019769, RRID:AB_1858329

Product name

Anti-TRIOBP

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

ATDSRTPEVPAGEGPRRGLGAPLTEDQQNRLSEEI
EKKWQELEKLPLRENKRVPLTALLNQSRGERRGPP
SDGHEALEKEVQALRAQLEAWRLQGEAPQSA


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Western blot analysis in human cell lines A-431 and Caco-2 using Anti-TRIOBP antibody. Corresponding TRIOBP RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.

Orthogonal method

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in human cell line U-87 MG.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human rectum shows strong cytoplasmic and membranous positivity in glandular cells.

Sample type

HUMAN