Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Immunohistochemistry [5]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-34745 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- UQCRFS1 Recombinant Rabbit Monoclonal Antibody (JG62-31)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: A549, 293, mouse kidney tissue lysate, 293T, LOVO, SiHa, rat skeletal muscle tissue, human colon cancer tissue, human appendix tissue, human kidney tissue, mouse cerebellum tissue.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JG62-31
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of UQCRFS1 in Lane 1: A549, Lane 2: 293, Lane 3: Mouse kidney. Samples were incubated with UQCRFS1 monoclonal antibody (Product # MA5-34745), at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of UQCRFS1 in SiHa cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with UQCRFS1 monoclonal antibody (Product # MA5-34745), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of UQCRFS1 in LOVO cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with UQCRFS1 monoclonal antibody (Product # MA5-34745), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of UQCRFS1 in 293T cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with UQCRFS1 monoclonal antibody (Product # MA5-34745), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of UQCRFS1 in paraffin-embedded rat skeletal muscle tissue. Samples were incubated with UQCRFS1 monoclonal antibody (Product # MA5-34745), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of UQCRFS1 in paraffin-embedded mouse cerebellum tissue. Samples were incubated with UQCRFS1 monoclonal antibody (Product # MA5-34745), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of UQCRFS1 in paraffin-embedded human kidney tissue. Samples were incubated with UQCRFS1 monoclonal antibody (Product # MA5-34745), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of UQCRFS1 in paraffin-embedded human colon cancer tissue. Samples were incubated with UQCRFS1 monoclonal antibody (Product # MA5-34745), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of UQCRFS1 in paraffin-embedded human appendix tissue. Samples were incubated with UQCRFS1 monoclonal antibody (Product # MA5-34745), and followed by hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of UQCRFS1 in A549 cells (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple). Samples were incubated with UQCRFS1 monoclonal antibody (Product # MA5-34745) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.