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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- PAB4186 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB4186, RRID:AB_1581819
- Product name
- USP7 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of USP7.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Human and mouse proteases: a comparative genomic approach.
Deubiquitination of p53 by HAUSP is an important pathway for p53 stabilization.
Deubiquitinating enzymes: a new class of biological regulators.
Puente XS, Sánchez LM, Overall CM, López-Otín C
Nature reviews. Genetics 2003 Jul;4(7):544-58
Nature reviews. Genetics 2003 Jul;4(7):544-58
Deubiquitination of p53 by HAUSP is an important pathway for p53 stabilization.
Li M, Chen D, Shiloh A, Luo J, Nikolaev AY, Qin J, Gu W
Nature 2002 Apr 11;416(6881):648-53
Nature 2002 Apr 11;416(6881):648-53
Deubiquitinating enzymes: a new class of biological regulators.
D'Andrea A, Pellman D
Critical reviews in biochemistry and molecular biology 1998;33(5):337-52
Critical reviews in biochemistry and molecular biology 1998;33(5):337-52
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The USP7 polyclonal antibody (Cat # PAB4186) is used in Western blot to detect USP7 in T-47D cell lysate .
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human testis tissue reacted with USP7 polyclonal antibody (Cat # PAB4186) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated .
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with the USP7 polyclonal antibody (Cat # PAB4186) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . HC = hepatocarcinoma .
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
