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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 600-401-482 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#600-401-482, RRID:AB_2214025
- Product name
- Anti-HAUSP (RABBIT) Antibody - 600-401-482
- Antibody type
- Polyclonal
- Vial size
- 100 µl
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Supportive validation
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- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western blot using Rockland's affinity purified anti-HAUSP antibody shows detection of HAUSP in various cell lysates at 130 kDa (lane 1 - HeLa nuclear extract, lane 2 - HeLa, Lane 3 - A431, Lane 4 - MCF7, Lane 5 - 3T3). The antibody is blocked by pre-incubation with the immunizing peptide (lanes 6 - 10) using the same lysates. A 1:500 dilution of the primary antibody was used for detection followed by a 1:5,000 dilution of HRP Gt-a-Rabbit IgG. Exposure time was 30 s.
- Validation comment
- Western Blot
