A700-072

antibody from Invitrogen Antibodies

Targeting: USP7 HAUSP

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A700-072

Provider

Invitrogen Antibodies

Product name

USP7/HAUSP Recombinant Rabbit Monoclonal Antibody (BLR072G)

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Antibody clone number

BLR072G

Vial size

100 µL

Concentration

250 µg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse USP7/HAUSP by western blot. Samples: Whole cell lysate (50 µg) from RKO, HEK293T, K-562, Hep-G2, MCF-7, Jurkat, HeLa, U2OS, NIH 3T3, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-USP7/HAUSP recombinant monoclonal antibody [BLR072G] (Product # A700-072 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-eEF2 (Product # A301-688A).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human USP7/HAUSP by immunocytochemistry.Sample: FFPE section of OVCAR-4 cells.Antibody: Rabbit anti-USP7/HAUSP recombinant monoclonal antibody [BLR072G] (Product # A700-072; lot 1) used at 1:250.Secondary: HRP-conjugated goat anti-rabbit IgG (Product # A120-501P). Substrate: DAB.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human USP7/HAUSP by immunohistochemistry. AntibodySample: FFPE section of prostate carcinoma.Antibody: Rabbit anti-USP7/HAUSP recombinant monoclonal antibody (BLR072G) (A700-072 lot 1) used at 1:250.Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of mouse USP7/HAUSP by immunohistochemistry.Sample: FFPE section of mouse renal cell carcinoma.Antibody: Rabbit anti-USP7/HAUSP recombinant monoclonal antibody [BLR072G] (Product # A700-072 lot 1) used at 1:250.Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human USP7/HAUSP by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-USP7/HAUSP recombinant monoclonal antibody [BLR072G] (Product # A700-072 lot 1) used for IP at 20 µL/mg lysate. USP7/HAUSP was also immunoprecipitated by rabbit anti-USP7 antibody (Product # A300-033A). For blotting immunoprecipitated USP7/HAUSP (Product # A700-072) was used at 1:1000. Chemiluminescence with an exposure time of 3 seconds.