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LearnPAB3245
antibody from Abnova Corporation
Targeting: BRD2
BRD2-IT1, D6S113E, FSRG1, KIAA9001, NAT, RING3
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PAB3245 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3245, RRID:AB_1571771
- Product name
- BRD2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of BRD2.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Therapeutic targeting of BET bromodomain proteins in castration-resistant prostate cancer.
BRD2 (RING3) is a probable major susceptibility gene for common juvenile myoclonic epilepsy.
Reproductive cycle regulation of nuclear import, euchromatic localization, and association with components of Pol II mediator of a mammalian double-bromodomain protein.
RING3 kinase transactivates promoters of cell cycle regulatory genes through E2F.
Asangani IA, Dommeti VL, Wang X, Malik R, Cieslik M, Yang R, Escara-Wilke J, Wilder-Romans K, Dhanireddy S, Engelke C, Iyer MK, Jing X, Wu YM, Cao X, Qin ZS, Wang S, Feng FY, Chinnaiyan AM
Nature 2014 Jun 12;510(7504):278-82
Nature 2014 Jun 12;510(7504):278-82
BRD2 (RING3) is a probable major susceptibility gene for common juvenile myoclonic epilepsy.
Pal DK, Evgrafov OV, Tabares P, Zhang F, Durner M, Greenberg DA
American journal of human genetics 2003 Aug;73(2):261-70
American journal of human genetics 2003 Aug;73(2):261-70
Reproductive cycle regulation of nuclear import, euchromatic localization, and association with components of Pol II mediator of a mammalian double-bromodomain protein.
Crowley TE, Kaine EM, Yoshida M, Nandi A, Wolgemuth DJ
Molecular endocrinology (Baltimore, Md.) 2002 Aug;16(8):1727-37
Molecular endocrinology (Baltimore, Md.) 2002 Aug;16(8):1727-37
RING3 kinase transactivates promoters of cell cycle regulatory genes through E2F.
Denis GV, Vaziri C, Guo N, Faller DV
Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research 2000 Aug;11(8):417-24
Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research 2000 Aug;11(8):417-24
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of BRD2 (arrow) using rabbit BRD2 polyclonal antibody (Cat # PAB3245). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BRD2 gene (Lane 2) (Origene Technologies).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- (LEFT) Western blot analysis of BRD2 polyclonal antibody (Cat # PAB3245) in A-375 cell lysate. BRD2 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with BRD2 polyclonal antibody (Cat # PAB3245) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
