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LearnNBP1-30475
antibody from Novus Biologicals
Targeting: BRD2
BRD2-IT1, D6S113E, FSRG1, KIAA9001, NAT, RING3
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- NBP1-30475 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-30475, RRID:AB_2065883
- Product name
- Rabbit Polyclonal BRD2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references miR-126-5p promotes retinal endothelial cell survival through SetD5 regulation in neurons.
Villain G, Poissonnier L, Noueihed B, Bonfils G, Rivera JC, Chemtob S, Soncin F, Mattot V
Development (Cambridge, England) 2018 Jan 8;145(1)
Development (Cambridge, England) 2018 Jan 8;145(1)
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BRD2 Antibody [NBP1-30475] - Whole cell lysate from HeLa and 293T cells. BRD2 was also immunoprecipitated by rabbit anti-BRD2 antibody NBP1-30474.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BRD2 Antibody [NBP1-30475] - Whole cell lysate (15 and 50 ug) from NIH3T3 cells. Antibody used at 0.2 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BRD2 Antibody [NBP1-30475] - Detection of Human and Mouse BRD2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRD2 antibody NBP1-30475 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: BRD2 Antibody [NBP1-30475] - Detection of human BRD2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BRD2 antibody NBP1-30475 used for IP at 6 ug per reaction. BRD2 was also immunoprecipitated by rabbit anti-BRD2 antibody NBP1-30474 and rabbit anti-BRD2 recombinant monoclonal antibody [BL-167-2A2] (NBP2-76410). For blotting immunoprecipitated BRD2, NBP2-76410 was used at 1:1000. Detection: Chemiluminescence with an exposure time time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: BRD2 Antibody [NBP1-30475] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-BRD2 used at a dilution of 1:50 (4ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BRD2 Antibody [NBP1-30475] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-BRD2 (NBP1-30475). Detection: DAB
