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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [6]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-78575 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EFHD2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Positive Control: H1299, HeLaS3, mouse brain, rat brain
- Concentration
- 0.2 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of EFHD2 in various tissue extracts using 50 µg of protein. Samples were separated with 12% SDS-PAGE and incubated with EFHD2 polyclonal antibody (Product # PA5-78575) using a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of EFHD2 in H1299 cells using 30 µg of protein. Samples were separated with 10% SDS-PAGE and incubated with EFHD2 polyclonal antibody (Product # PA5-78575) using a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using EFHD2 Polyclonal Antibody (Product # PA5-78575). Various tissue extracts (50 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with EFHD2 Polyclonal Antibody (Product # PA5-78575) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of EFHD2 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a EFHD2 Polyclonal Antibody (Product # PA5-78575) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using EFHD2 Polyclonal Antibody (Product # PA5-78575). Sample (30 µg of whole cell lysate). A: H1299. 10% SDS PAGE. EFHD2 Polyclonal Antibody (Product # PA5-78575) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using EFHD2 Polyclonal Antibody (Product # PA5-78575). Various tissue extracts (50 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with EFHD2 Polyclonal Antibody (Product # PA5-78575) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- EFHD2 Polyclonal Antibody detects EFHD2 protein by immunofluorescent analysis. Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: EFHD2 protein stained by EFHD2 Polyclonal Antibody (Product # PA5-78575) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta-3 Tubulin Polyclonal Antibody [GT1338]diluted at 1:500. Blue: Fluoroshield with DAPI .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- EFHD2 Polyclonal Antibody detects EFHD2 protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: EFHD2 protein stained by EFHD2 Polyclonal Antibody (Product # PA5-78575) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500. Blue: Fluoroshield with DAPI .
