AP8433b

antibody from Abgent

Targeting: PPP1CB MP, PP-1B, PP1B, PP1beta, PP1c, PPP1beta

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP8433b

Provider

Abgent

Proper citation

Abgent Cat#AP8433b, RRID:AB_637339

Product name

Protein Phosphatase 1 beta (PPP1CB) Antibody (C-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

2 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of PPP1CB (arrow) using rabbit polyclonal PPP1CB Antibody (K301) (Cat. #AP8433b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PPP1CB gene.

Primary Ab dilution

1:1000

Immunocytochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Fluorescent image of U251 cell stained with PPP1CB Antibody (C-term)(Cat#AP8433b).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PPP1CB primary antibody (1:25, 1 h at 37!). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37!).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37!).PPP1CB immunoreactivity is localized to Cytoplasm and Nucleus significantly. "

Primary Ab dilution

1:10~50