GTX105295

antibody from GeneTex

Targeting: PPP1CB MP, PP-1B, PP1B, PP1beta, PP1c, PPP1beta

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX105295

Provider

GeneTex

Proper citation

GeneTex Cat#GTX105295, RRID:AB_1951348

Product name

PPP1CB antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ?g whole cell lysate)A: 293TB: A431 (GTX27909)C: H1299D: HeLa S3 (GTX14654)E: HepG2 (GTX27900)F: MOLT4 (GTX27912)G: Raji (GTX27908) 7.5% SDS PAGEGTX105295 diluted at 1:1000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (50 ?g of whole cell lysate) A: Mouse brain 10% SDS PAGE GTX105295 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with PPP1CB antibody (GTX105295) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

PPP1CB antibody detects PPP1CB protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde for 10 min.Green: PPP1CB protein stained by PPP1CB antibody (GTX105295) diluted at 1:100.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

PPP1CB antibody detects PPP1CB protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PPP1CB stained by PPP1CB antibody (GTX105295) diluted at 1:500.Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

PPP1CB antibody detects PPP1CB protein at nucleus and cytosol on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. PPP1CB antibody (GTX105295) dilution: 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

PPP1CB antibody detects PPP1CB protein at nucleus and cytosol on mouse hind brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse hind brain. PPP1CB antibody (GTX105295) dilution: 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

PPP1CB antibody detects PPP1CB Protein expression by immunohistochemical analysis.Sample: Frozen-sectioned adult mouse cerebellum. Green: PPP1CB stained by PPP1CB antibody (GTX105295) diluted at 1:250.Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).