- NBP2-76886 - Novus Biologicals PPP1CB antibody

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Supportive validation

Submitted by: Novus Biologicals (provider)
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Experimental details: Western Blot: Protein Phosphatase 1 beta Antibody (JE45-08) [NBP2-76886] - Western blot analysis of PPP1CB on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse skeletal muscle tissue lysate Lane 2: Rat brain tissue lysate Lane 3: A431 cell lysate Lane 4: SH-SY-5Y cell lysate Lane 5: SiHa cell lysate Lane 6: 293 cell lysate Lane 7: Hela cell lysate Lane 8: PC-3M cell lysate Lane 9: HepG2 cell lysate Lane 10: A549 cell lysate

Supportive validation

Submitted by: Novus Biologicals (provider)
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Experimental details: Immunohistochemistry: Protein Phosphatase 1 beta Antibody (JE45-08) [NBP2-76886] - Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PPP1CB antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 mins.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry: Protein Phosphatase 1 beta Antibody (JE45-08) [NBP2-76886] - Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-PPP1CB antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 mins.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry: Protein Phosphatase 1 beta Antibody (JE45-08) [NBP2-76886] - Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-PPP1CB antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 mins.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry: Protein Phosphatase 1 beta Antibody (JE45-08) [NBP2-76886] - Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-PPP1CB antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 mins.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

NBP2-76886