Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
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Validation data
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- Product number
- H00030012-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00030012-M01, RRID:AB_548713
- Product name
- Mouse Monoclonal TLX3 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. TLX3 - T-cell leukemia homeobox 3
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TLX3 Antibody (2A3) [H00030012-M01] - Analysis of TLX3 over-expressed 293 cell line, cotransfected with TLX3 Validated Chimera RNAi ( Cat # H00030012-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TLX3 monoclonal antibody (M01), clone 2A3 (Cat # H00030012-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TLX3 Antibody (2A3) [H00030012-M01] - Analysis of TLX3 expression in transfected 293T cell line by TLX3 monoclonal antibody (M01), clone 2A3.Lane 1: TLX3 transfected lysate(31.9 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TLX3 Antibody (2A3) [H00030012-M01] - TLX3 monoclonal antibody (M01), clone 2A3 Analysis of TLX3 expression in MCF-7.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: TLX3 Antibody (2A3) [H00030012-M01] - Detection limit for recombinant GST tagged TLX3 is approximately 0.03ng/ml as a capture antibody.