STJ11101322
antibody from St John's Laboratory
Targeting: CTNND1
CTNND, KIAA0384, p120, p120cas, p120ctn
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Chromatin Immunoprecipitation [1]
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Validation data
Reference
Comment
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- Product number
- STJ11101322 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-CTNND1 antibody [ARC0586] (STJ11101322)
- Antibody type
- Monoclonal
- Description
- Rabbit monoclonal antibody anti-Delta-Catenin/p120 Catenin is suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
NA
- Epitope
- NA
- Isotype
- IgG
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- Store in a freezer at-20°C and avoid freeze-thaw cycles.
- Handling
- NA
No comments: Submit comment
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using Delta-Catenin/p120 Catenin rabbit monoclonal antibody (STJ11101322) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human esophageal using Delta-Catenin/p120 Catenin rabbit monoclonal antibody (STJ11101322) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of 600ug extracts of mouse testis cells using 3ug Delta-Catenin/p120 Catenin antibody (STJ11101322). Western blot was performed from the immunoprecipitate using Delta-Catenin/p120 Catenin antibody (STJ11101322) at a dilition of 1:1000.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA