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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- Ab099575 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Recombinant delta 1 Catenin/CAS Antibody
- Antibody type
- Recombinant
- Description
- Rabbit anti Human delta 1 Catenin/CAS Antibody, Recombinant, could be used for WB, IHC and so on.Application_WB: 1/500 Ð 1/1000IHC: 1/50-1/100Protein FunctionBinds to and inhibits the transcriptional repressor ZBTB33, which may lead to activation of target genes of the Wnt signaling pathway (By similarity). May associate with and regulate the cell adhesion properties of both C- and E-cadherins. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. Promotes GLIS2 C-terminal cleavage.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 955-968- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 0,2 mg/ml
- Storage
- Store at 4_ short term (1-2 weeks). Store at -20_ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant delta 1 CateninCAS Antibody (Ab099575) - Western Blot All lanes: Recombinant delta 1 CateninCAS Antibody (Ab099575) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 108 kDa Observed band size: 63, 68, 91,108 kDa Exposure time: 4.5 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant delta 1 CateninCAS Antibody (Ab099575) - IHC Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Recombinant delta 1 CateninCAS Antibody (Ab099575). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
