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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AP54007PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP54007PU-N, RRID:AB_11147104
- Product name
- anti Speedy-1 / RINGO A (Center)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide - KLH conjugated - corresponding to the central region (between 154-183aa) of human Speedy-1 / RINGO A
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.1 mg
- Concentration
- 0.25 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of Speedy-1 / RINGO (arrow) A in K562 cell line lysates (35ug/lane) using Speedy-1 / RINGO A antibody Cat.-No. AP54007PU-N.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of Speedy-1 / RINGO A (arrow) using Speedy-1 / RINGO A antibody Cat.-No. AP54007PU-N: 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SPDYA gene.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in prostate carcinoma (Formalin-fixed, Paraffin-embedded) using Speedy-1 / RINGO A antibody Cat.-No. AP54007PU-N, followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SPDYA antibody for IHC; Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram) using Speedy-1 / RINGO A antibody Cat.-No. AP54007PU-N, followed by FITC-conjugated goat-anti-rabbit secondary antibodies.
