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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NB100-2521 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-2521, RRID:AB_10000728
- Product name
- Rabbit Polyclonal Speedy/Ringo Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat, Sheep
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.1 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Spy1, a unique cell cycle regulator, alters viability in ALS motor neurons and cell lines in response to mutant SOD1-induced DNA damage.
Cell adhesion to fibronectin down-regulates the expression of Spy1 and contributes to drug resistance in multiple myeloma cells.
The cyclin-like protein Spy1/RINGO promotes mammary transformation and is elevated in human breast cancer.
IL-1beta, IL-6, and RANTES as biomarkers of Chikungunya severity.
The cyclin-dependent kinase activator, Spy1A, is targeted for degradation by the ubiquitin ligase NEDD4.
Increased activation of stromal interaction molecule-1/Orai-1 in aorta from hypertensive rats: a novel insight into vascular dysfunction.
CDC2/SPDY transiently associates with endoplasmic reticulum exit sites during oocyte maturation.
Wang XD, Zhu MW, Shan D, Wang SY, Yin X, Yang YQ, Wang TH, Zhang CT, Wang Y, Liang WW, Zhang J, Jiang HZ, Dong GT, Jiang HQ, Qi Y, Feng HL
DNA repair 2019 Feb;74:51-62
DNA repair 2019 Feb;74:51-62
Cell adhesion to fibronectin down-regulates the expression of Spy1 and contributes to drug resistance in multiple myeloma cells.
Fei M, Hang Q, Hou S, Ruan C
International journal of hematology 2013 Oct;98(4):446-55
International journal of hematology 2013 Oct;98(4):446-55
The cyclin-like protein Spy1/RINGO promotes mammary transformation and is elevated in human breast cancer.
Al Sorkhy M, Ferraiuolo RM, Jalili E, Malysa A, Fratiloiu AR, Sloane BF, Porter LA
BMC cancer 2012 Jan 26;12:45
BMC cancer 2012 Jan 26;12:45
IL-1beta, IL-6, and RANTES as biomarkers of Chikungunya severity.
Ng LF, Chow A, Sun YJ, Kwek DJ, Lim PL, Dimatatac F, Ng LC, Ooi EE, Choo KH, Her Z, Kourilsky P, Leo YS
PloS one 2009;4(1):e4261
PloS one 2009;4(1):e4261
The cyclin-dependent kinase activator, Spy1A, is targeted for degradation by the ubiquitin ligase NEDD4.
Al Sorkhy M, Craig R, Market B, Ard R, Porter LA
The Journal of biological chemistry 2009 Jan 30;284(5):2617-27
The Journal of biological chemistry 2009 Jan 30;284(5):2617-27
Increased activation of stromal interaction molecule-1/Orai-1 in aorta from hypertensive rats: a novel insight into vascular dysfunction.
Giachini FR, Chiao CW, Carneiro FS, Lima VV, Carneiro ZN, Dorrance AM, Tostes RC, Webb RC
Hypertension (Dallas, Tex. : 1979) 2009 Feb;53(2):409-16
Hypertension (Dallas, Tex. : 1979) 2009 Feb;53(2):409-16
CDC2/SPDY transiently associates with endoplasmic reticulum exit sites during oocyte maturation.
Hölzenspies JJ, Stoorvogel W, Colenbrander B, Roelen BA, Gutknecht DR, van Haeften T
BMC developmental biology 2009 Feb 3;9:8
BMC developmental biology 2009 Feb 3;9:8
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Speedy/Ringo Antibody [NB100-2521] - Detection of Speedy/Ringo in mouse testis (2ug/ml).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Speedy/Ringo Antibody [NB100-2521] - Speedy/Ringo was detected in immersion fixed paraffin-embedded sections of human testis using Rabbit Anti-Human Speedy/Ringo polyclonal Antibody (Catalog # NB100-2521) at 1:300 for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nuclei of sperm cells.
