Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
- Other assay [1]
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- Product number
- PA5-83607 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NCBP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references 2'-O-Methylation of the second transcribed nucleotide within the mRNA 5' cap impacts the protein production level in a cell-specific manner and contributes to RNA immune evasion.
Drazkowska K, Tomecki R, Warminski M, Baran N, Cysewski D, Depaix A, Kasprzyk R, Kowalska J, Jemielity J, Sikorski PJ
Nucleic acids research 2022 Sep 9;50(16):9051-9071
Nucleic acids research 2022 Sep 9;50(16):9051-9071
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NCBP1 by a NCBP1 polyclonal antibody (Product # PA5-83607). Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Human cell line RT-4.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of NCBP1 in A-431 cells using a NCBP1 polyclonal antibody (Product # PA5-83607). The analysis shows localization to nucleoplasm & cytosol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NCBP1 in human pancreas using a NCBP1 polyclonal antibody (Product # PA5-83607). The analysis shows low expression as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NCBP1 in human testis and pancreas tissues using a NCBP1 polyclonal antibody (Product # PA5-83607). Corresponding RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NCBP1 in human testis using a NCBP1 polyclonal antibody (Product # PA5-83607). The analysis shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NCBP1 in human pancreas using a NCBP1 polyclonal antibody (Product # PA5-83607). The analysis shows low expression as expected.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5. Methylation of the first transcribed nucleotides in mRNA only partially prevents protein level decrease under IFN-induced stress. Relative protein production levels 72 h after a 5 h IFNalpha pre-treatment in ( A and B ) A549 and ( C and D ) JAWS II cells. Data were analysed for three independent replicates (each experiment consisted of three technical replicates). Bars for each transcript represent the mean value +- SEM normalized to protein production in mock-treated cells. Statistical significance: * P