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LearnAP09238PU-N
antibody from Acris Antibodies GmbH
Targeting: FBXW11
BTRC2, BTRCP2, Fbw11, Fbw1b, FBXW1B, Hos, KIAA0696
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- AP09238PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP09238PU-N, RRID:AB_2035471
- Product name
- anti FBW1B
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide corresponding to a region near the N-terminal of human betaTrCP2 protein
- Reactivity
- Human, Mouse, Bovine, Canine, Chicken/Avian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
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- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot using affinity purified anti-betaTrCP2 antibody shows detection of mouse and human betaTrCP2 (arrowhead) in NIH3T3 (lane 1) and 293 (lane 2) whole cell lysates, respectively. The band appears as a 58 kDa protein, although a 62.1 kDa band is predicted. The identity of faint higher molecular weight bands is not known. The primary antibody was used at a 1:200 dilution incubated in 5% BLOTTO overnight at 4°C. Detection occurred using HRP conjugated Goat-anti-Rabbit IgG diluted 1:20,000 in blocking buffer for 1 h at 4 °C.
