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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00003115-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00003115-M01, RRID:AB_425481
- Product name
- HLA-DPB1 monoclonal antibody (M01), clone 6C6
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant HLA-DPB1.
- Antigen sequence
MMVLQVSAAPRTVALTALLMVLLTSVVQGRATPEN
YVYQGRQECYAFNGTQRFLERYIYNREEYARFDSD
VGEFRAVTELGRPAAEYWNSQKDILEEKRAVPDRV
CRHNYELDEAVTLQRRVQPKVNVSPSKKGPLQHHN
LLVCHVTDFYPGSIQVRWFLNGQEETAGVVSTNLI
RNGDWTFQILVMLEMTPQQGDVYICQVEHTSLDSP
VTVEWKAQSDSAQSKTLTGAGGFVLGLIICGVGIF
MHRRSKKVQRGSA- Isotype
- IgG
- Antibody clone number
- 6C6
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of HLA-DPB1 expression in transfected 293T cell line by HLA-DPB1 monoclonal antibody (M01), clone 6C6.Lane 1: HLA-DPB1 transfected lysate(29 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged HLA-DPB1 is 10 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of HLA-DPB1 transfected lysate using anti-HLA-DPB1 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with HLA-DPB1 monoclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to HLA-DPB1 on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 0.5 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
