Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00003115-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00003115-M01, RRID:AB_540502
- Product name
- Mouse Monoclonal HLA DPB1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. HLA-DPB1 - major histocompatibility complex, class II, DP beta 1
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HLA DPB1 Antibody (6C6) [H00003115-M01] - Analysis of HLA-DPB1 expression in transfected 293T cell line by HLA-DPB1 monoclonal antibody (M01), clone 6C6.Lane 1: HLA-DPB1 transfected lysate(29 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: HLA DPB1 Antibody (6C6) [H00003115-M01] - Detection limit for recombinant GST tagged HLA-DPB1 is 10 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: HLA DPB1 Antibody (6C6) [H00003115-M01] - Analysis of HLA-DPB1 transfected lysate using anti-HLA-DPB1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HLA-DPB1 monoclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HLA DPB1 Antibody (6C6) [H00003115-M01] - Analysis of monoclonal antibody to HLA-DPB1 on formalin-fixed paraffin-embedded human lymph node. Antibody concentration 0.5 ug/ml.