V7585

antibody from NSJ Bioreagents

Targeting: HLA-DPB1 HLA-DP1B

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Protein array (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

V7585

Provider

NSJ Bioreagents

Product name

Pan-HLA Antibody (MHC II)

Antibody type

Monoclonal

Description

Protein A affinity chromatography

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Vial size

20 µg, 100 µg (with BSA, sodium azide), 100 µg (without BSA, sodium azide), 7 ml (prediluted for IHC)

Concentration

0.2 mg/ml, 1 mg/ml (BSA/azide free format), Prediluted (IHC only format)

Storage

Store the Pan-HLA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human Ramos lysate with Pan-HLA antibody (clone HLA-Pan/2967R). Expected molecular weight: 33-35 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescence staining of human Raji cells with Pan-HLA antibody (green, clone HLA-Pan/2967R) and Reddot nuclear stain (red).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescence staining of human Ramos cells with Pan-HLA antibody (green, clone HLA-Pan/2967R) and Reddot nuclear stain (red).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human tonsil tissue with recombinant Pan-HLA antibody (clone HLA-Pan/2967R). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min and allow to cool prior to testing.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human Ramos cells with Pan-HLA antibody (clone HLA-Pan/2967R); Red=isotype control, Blue= Pan-HLA antibody.

Protein array

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Pan-HLA antibody (clone HLA-Pan/2967R). These results demonstrate the foremost specificity of the HLA-Pan/2967R mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Other assay

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

SDS-PAGE analysis of purified, BSA-free recombinant Pan-HLA antibody (clone HLA-Pan/2967R) as confirmation of integrity and purity.