MAB6910

antibody from Abnova Corporation

Targeting: ENTPD1 ATPDase, CD39, NTPDase-1, SPG64

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB6910

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#MAB6910, RRID:AB_10679084

Product name

ENTPD1 monoclonal antibody, clone BU61

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against native ENTPD1.

Isotype

IgG

Antibody clone number

BU61

Storage

Store at 4°C.

References

Submitted references

The CD39 lymphoid cell activation antigen. Molecular cloning and structural characterization.

Maliszewski CR, Delespesse GJ, Schoenborn MA, Armitage RJ, Fanslow WC, Nakajima T, Baker E, Sutherland GR, Poindexter K, Birks C
Journal of immunology (Baltimore, Md. : 1950) 1994 Oct 15;153(8):3574-83

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Cloning and sequencing of a trophoblast-endothelial-activated lymphocyte surface protein: cDNA sequence and genomic structure.

Voland JR, Wyzykowski RJ, Huang M, Dutton RW
Proceedings of the National Academy of Sciences of the United States of America 1992 Nov 1;89(21):10425-9

Flow cytometry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

5 X 10⁵ ficoll prepared human peripheral blood lymphocytes were washed and incubated 45 minutes on ice with 80 ml of anti-CD39 (FITC) at a 1 : 50 dilution. Cells were washed three times, fixed and analyzed on a BD FACstar plus. A net 2.2% population of the cells stained positive with a mean shift of 1.25 log10 fluorescent units when compared to a Mouse IgG1/FITC negative control at a similar concentration. Binding was 100% blocked when cells were pre incubated 10 minutes with 20 ml of 0.5 mg/mL ENTPD1 monoclonal antibody, clone BU61 (Cat # MAB6910).

Validation comment

Flow Cytometry