PA5-118833

DOK4 antibody from Invitrogen Antibodies

FLJ10488

Western blot

Supportive data in Antibodypedia

Immunocytochemistry

Supportive data in Antibodypedia

Immunohistochemistry

Supportive data in Antibodypedia

Provider product page for PA5-118833

Antibody Data

Product number

PA5-118833

Provider

Invitrogen Antibodies

Product name

DOK4 Polyclonal Antibody

Provider product page

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Applications Reported: This eBioGoH3 antibody has been reported for use in flow cytometric analysis.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DOK4 in mouse muscle using a DOK4 polyclonal antibody (Product # PA5-118833).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of DOK4 in 293 cells using a DOK4 polyclonal antibody (Product # PA5-118833). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Red), diluted at 1:600, was used as secondary antibody.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of DOK4 in Mouse heart tissue using a DOK4 polyclonal antibody (Product # PA5-118833). The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of DOK4 in Rat testis tissue using a DOK4 polyclonal antibody (Product # PA5-118833). The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of DOK4 in Rat muscle tissue using a DOK4 polyclonal antibody (Product # PA5-118833). The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of DOK4 in Rat skin tissue using a DOK4 polyclonal antibody (Product # PA5-118833). The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.