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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
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- Product number
- MA5-31478 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- COL11A2 Monoclonal Antibody (GT212)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Keep as concentrated solution. Predicted reactivity: Mouse (89%), Rat (89%), Bovine (93%). Positive Control: human COLC11A2-transfected 293T cells (partial fragment). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- GT212
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of COL11A2 in A) 293T whole cell lysate, B) whole cell lysate of human COLC11A2-transfected 293T cells (partial fragment) using COL11A2 monoclonal antibody (Product # MA5-31478) using 30 µg of sample at a dilution of 1:10000. Prior to incubation with primary antibody, the sample was separated on 12% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of COL11A2 was performed by separating 50 µg of various tissue extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a COL11A2 Monoclonal Antibody (GT212) (Product # MA5-31478) at a dilution of 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- COL11A2 Monoclonal Antibody (GT212) detects COL11A2 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg whole cell lysate/extract of human COLC11A2-transfected 293T cells (partial fragment).12 % SDS-PAGE. COL11A2 Monoclonal Antibody (GT212) (Product # MA5-31478) dilution: 1:10,000.
