GTX54748

antibody from GeneTex

Targeting: EIF1 A121, EIF-1, EIF1A, ISO1, SUI1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX54748

Provider

GeneTex

Product name

eIF1 antibody [2B9]

Antibody type

Monoclonal

Reactivity

Human, Mouse, Rat

Host

Mouse

References

Submitted references

DHX29 reduces leaky scanning through an upstream AUG codon regardless of its nucleotide context.

Pisareva VP, Pisarev AV
Nucleic acids research 2016 May 19;44(9):4252-65

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

WB analysis of various cell lysates (80£gg per lane) using eIF1 antibody [2B9] at a dilution of 1:1000.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

ICC/IF analysis of HeLa and NIH3T3 cells using eIF1 antibody [2B9] (green) at a dilution of 1:50. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

ICC/IF analysis of HeLa cells with (right) or without (left) eIF1 antibody [2B9] at a dilution of 1:200 (green). F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

ICC/IF analysis of MCF7 cells with (right) or without (left) eIF1 antibody [2B9] at a dilution of 1:200 (green).. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

ICC/IF analysis of NIH-3T3 cells with (right) or without (left) eIF1 antibody [2B9] at a dilution of 1:200 (green). F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI.