H00000306-M12

antibody from Abnova Corporation

Targeting: ANXA3 ANX3

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00000306-M12

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00000306-M12, RRID:AB_605929

Product name

ANXA3 monoclonal antibody (M12), clone 4F1

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant ANXA3.

Antigen sequence

MASIWVGHRGTVRDYPDFSPSVDAEAIQKAIRGIG
TDEKMLISILTERSNAQRQLIVKEYQAAYGKELKD
DLKGDLSGHFEHLMVALVTPPAVFDAKQLKKSMKG
AGTNEDALIEILTTRTSRQMKDISQAYYTVYKKSL
GDDISSETSGDFRKALLTLADGRRDESLKVDEHLA
KQDAQILYKAGENRWGTDEDKFTEILCLRSFPQLK
LTFDEYRNISQKDIVDSIKGELSGHFEDLLLAIVN
CVRNTPAFLAERLHRALKGIGTDEFTLNRIMVSRS
EIDLLDIRTEFKKHYGYSLYSAIKSDTSGDYEITL
LKICGGDD


Isotype

IgG

Antibody clone number

4F1

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Annexin A3 as a negative regulator of adipocyte differentiation.

Watanabe T, Ito Y, Sato A, Hosono T, Niimi S, Ariga T, Seki T
Journal of biochemistry 2012 Oct;152(4):355-63

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A multiplex model of combining gene-based, protein-based, and metabolite-based with positive and negative markers in urine for the early diagnosis of prostate cancer.

Cao DL, Ye DW, Zhang HL, Zhu Y, Wang YX, Yao XD
The Prostate 2011 May 15;71(7):700-10

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of ANXA3 expression in transfected 293T cell line by ANXA3 monoclonal antibody (M12), clone 4F1.Lane 1: ANXA3 transfected lysate(37.145 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged ANXA3 is approximately 1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol