Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF3444 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Neurogenin-3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Neurogenin-3 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse Neurogenin-3 is observed, and less than 1% cross-reactivity with recombinant human Neurogenin-1 is observed.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
Q9Y4Z2- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Wnt Signaling Separates the Progenitor and Endocrine Compartments during Pancreas Development.
YAP inhibition enhances the differentiation of functional stem cell-derived insulin-producing β cells.
Differentiation of human pluripotent stem cells into two distinct NKX6.1 populations of pancreatic progenitors.
Phosphorylation of NEUROG3 Links Endocrine Differentiation to the Cell Cycle in Pancreatic Progenitors.
An Activating STAT3 Mutation Causes Neonatal Diabetes through Premature Induction of Pancreatic Differentiation.
Prospectively isolated NGN3-expressing progenitors from human embryonic stem cells give rise to pancreatic endocrine cells.
The SDF-1α/CXCR4 axis is required for proliferation and maturation of human fetal pancreatic endocrine progenitor cells.
Sharon N, Vanderhooft J, Straubhaar J, Mueller J, Chawla R, Zhou Q, Engquist EN, Trapnell C, Gifford DK, Melton DA
Cell reports 2019 May 21;27(8):2281-2291.e5
Cell reports 2019 May 21;27(8):2281-2291.e5
YAP inhibition enhances the differentiation of functional stem cell-derived insulin-producing β cells.
Rosado-Olivieri EA, Anderson K, Kenty JH, Melton DA
Nature communications 2019 Apr 1;10(1):1464
Nature communications 2019 Apr 1;10(1):1464
Differentiation of human pluripotent stem cells into two distinct NKX6.1 populations of pancreatic progenitors.
Aigha II, Memon B, Elsayed AK, Abdelalim EM
Stem cell research & therapy 2018 Apr 3;9(1):83
Stem cell research & therapy 2018 Apr 3;9(1):83
Phosphorylation of NEUROG3 Links Endocrine Differentiation to the Cell Cycle in Pancreatic Progenitors.
Krentz NAJ, van Hoof D, Li Z, Watanabe A, Tang M, Nian C, German MS, Lynn FC
Developmental cell 2017 Apr 24;41(2):129-142.e6
Developmental cell 2017 Apr 24;41(2):129-142.e6
An Activating STAT3 Mutation Causes Neonatal Diabetes through Premature Induction of Pancreatic Differentiation.
Saarimäki-Vire J, Balboa D, Russell MA, Saarikettu J, Kinnunen M, Keskitalo S, Malhi A, Valensisi C, Andrus C, Eurola S, Grym H, Ustinov J, Wartiovaara K, Hawkins RD, Silvennoinen O, Varjosalo M, Morgan NG, Otonkoski T
Cell reports 2017 Apr 11;19(2):281-294
Cell reports 2017 Apr 11;19(2):281-294
Prospectively isolated NGN3-expressing progenitors from human embryonic stem cells give rise to pancreatic endocrine cells.
Cai Q, Bonfanti P, Sambathkumar R, Vanuytsel K, Vanhove J, Gysemans C, Debiec-Rychter M, Raitano S, Heimberg H, Ordovas L, Verfaillie CM
Stem cells translational medicine 2014 Apr;3(4):489-99
Stem cells translational medicine 2014 Apr;3(4):489-99
The SDF-1α/CXCR4 axis is required for proliferation and maturation of human fetal pancreatic endocrine progenitor cells.
Kayali AG, Lopez AD, Hao E, Hinton A, Hayek A, King CC
PloS one 2012;7(6):e38721
PloS one 2012;7(6):e38721
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Neurogenin-3 by Western Blot. Western blot shows lysates of human placenta and prostate tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Neurogenin-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3444) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Neurogenin-3 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
