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ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunoprecipitation [1]
- Other assay [4]
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- Product number
- GTX629701 - Provider product page
- Provider
- GeneTex
- Product name
- IQGAP1 antibody [EPR5221]
- Antibody type
- Monoclonal
- Host
- Rabbit
- Vial size
- 100 µl
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Supportive validation
- Submitted by
- GeneTex (provider)
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- Experimental details
- GTX629701 5-Hydroxymethylcytosine/ 5-hmC antibody [GT513] in MeDIP experiment. Human genomic DNA (500 ng) mixed with methylated cytosine standard kit (GTX400004) were subjected to MeDIP with 5-Hydroxymethylcytosine/ 5-hmC antibody [GT513] (GTX629701) at dilution of 1:500 and analyzed with semi-quantitative PCR. The immunoprecipitated DNA was plotted as % of input DNA.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Dotblot analysis of anti- 5-Hydroxymethylcytosine / 5-hmC antibody [GT513] with the synthetic DNA controls (GTX400004). DNA samples (2 to 50 ng as indicated) were spotted onto the positively charged Nylon membrane and blotted with 5-Hydroxymethylcytosine / 5-hmC antibody [GT513] (GTX629701) at a dilution of 1:500. A: Unmethylated DNA B: DNA containing 5-methylcytosine C: DNA containing 5-hydroxymethylcytosine
- Validation comment
- Dot
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Dotblot analysis of anti- 5-Hydroxymethylcytosine / 5-hmC antibody [GT513] with DNA samples DNA samples (50 to 500 ng as indicated) were spotted onto positively charged nylon membrane and blotted with 5-Hydroxymethylcytosine / 5-hmC antibody [GT513] (GTX629701) at a dilution of 1:500. A: Genomic DNA derived from 293T cells. B: Genomic DNA derived from human TET-1 transfected 293T cells.
- Validation comment
- Dot
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GTX629701 5-hydroxymethylcytosine antibody [GT513] Dot blot analysis of anti-5-hmC antibody with the synthetic DNA controls (GTX400004). DNA samples (0.5 to 50 ng as indicated) were spotted onto the positively charged Nylon membrane and blotted with 5-hmC antibody (GTX629701) at a dilution of 1:500. A: Unmethylated DNA fragment B: DNA fragment containing 5-methylcytosine C: DNA fragment containing 5-hydroxymethylcytosine D: DNA fragment containing 5-formylcytosine E: DNA fragment containing 5-carboxylcytosine
- Validation comment
- Dot
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GTX629701 5-Hydroxymethylcytosine / 5-hmC antibody [GT513] Dot blot analysis of anti-5-Hydroxymethylcytosine / 5-hmC antibody with the synthetic DNA controls (GTX400004). DNA samples (0.5 to 50 ng as indicated) were spotted onto the positively charged Nylon membrane and blotted with 5-Hydroxymethylcytosine / 5-hmC antibody [GT513] (GTX629701) at a dilution of 1:500. A: Unmethylated DNA B: DNA containing 5-methylcytosine C: DNA containing 5-hydroxymethylcytosine D: DNA containing 5-formylcytosine E: DNA containing 5-carboxylcytosine
- Validation comment
- Dot
