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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AF5069 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IQGAP1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects endogenous human IQGAP1 in Western blots.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
P46940- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human IQGAP1 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells, HepG2 human hepatocellular carcinoma cell line, U937 human histiocytic lymphoma cell line, and MRC-5 human embryonic lung fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human IQGAP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5069) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IQGAP1 at approximately 195 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
