Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PA5-95974 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ANT Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Immunogen sequence: LGGVDRHKQF WRYFAGNLAS GGAAGATSLC FVYPLDFART RLAADVGKGA AQREFHGLGD CIIKIFKSDG LRGLYQGFNV SVQGIIIYRA AYFGVYDTAK G
- Concentration
- 2.33 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using SLC25A4 Polyclonal antibody (Product # PA5-95974). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ANT in extracts of various cell lines using ANT Polyclonal Antibody (Product # PA5-95974) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of ANT was performed in HeLa cells using ANT Polyclonal Antibody (Product # PA5-95974) at a dilution of 1:100. Blue: DAPI for nuclear staining.