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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [6]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX105711 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX105711, RRID:AB_1950999
- Product name
- Nkx2.5 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample(30 ug whole cell lysate)A:H1299B:Raji (GTX27908)10% SDS PAGEGTX105711 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ug of whole cell lysate) A: mouse heart 10% SDS PAGE GTX105711 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Nkx2.5 antibody detects NKX2-5 protein by Western blot analysis.A. 50 µg rat heart lysate/extract10 % SDS-PAGENkx2.5 antibody (GTX105711) dilution: 1:500
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various tissue extracts (50 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with Nkx2.5 antibody (GTX105711) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with Nkx2.5 antibody (GTX105711) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various tissue extracts (50 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with Nkx2.5 antibody (GTX105711) diluted at 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC D3, using Nkx2.5(GTX105711) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Nkx2.5 antibody detects Nkx2.5 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat heart.Nkx2.5 stained by Nkx2.5 antibody (GTX105711) diluted at 1:500.
