R35130

antibody from NSJ Bioreagents

Targeting: NKX2-5 CSX, CSX1, NKX2.5, NKX2E, NKX4-1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

R35130

Provider

NSJ Bioreagents

Product name

NKX2.5 Antibody

Antibody type

Polyclonal

Antigen

Amino acids PRAYSDPDPAKDPR were used as the immunogen for this NKX2.5 antibody.

Description

Antigen affinity purified antibody

Reactivity

Human, Mouse, Rat

Host

Goat

Conjugate

Unconjugated

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

Aliquot and store the NKX2.5 antibody at -20°C.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human heart lysate with NKX2.5 antibody at 0.3ug/ml. Predicted molecular weight ~35kDa, routinely observed at 35~45kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human heart tissue with NKX2.5 antibody at 3.75ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.