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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NBP1-51953 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-51953, RRID:AB_11003900
- Product name
- Goat Polyclonal NKX2.5 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is expected to recognize all three reported isoforms (NP_004378.1
- Reactivity
- Human, Mouse
- Host
- Goat
- Antigen sequence
C-PRAYSDPDPAKDPR- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Perinatal loss of Nkx2-5 results in rapid conduction and contraction defects.
Briggs LE, Takeda M, Cuadra AE, Wakimoto H, Marks MH, Walker AJ, Seki T, Oh SP, Lu JT, Sumners C, Raizada MK, Horikoshi N, Weinberg EO, Yasui K, Ikeda Y, Chien KR, Kasahara H
Circulation research 2008 Sep 12;103(6):580-90
Circulation research 2008 Sep 12;103(6):580-90
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NKX2.5 Antibody [NBP1-51953] - (0.3ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NKX2.5 Antibody [NBP1-51953] - Staining of Mouse Heart lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NKX2.5 Antibody [NBP1-51953] - Staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
