Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- MAB2444 - Provider product page
- Provider
- R&D Systems
- Product name
- Human NKX2.5 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human NKX-2.5 in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P52952
- Isotype
- IgG
- Antibody clone number
- 259416
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references The involvement of protein kinase C-ε in isoflurane induced preconditioning of human embryonic stem cell--derived Nkx2.5(+) cardiac progenitor cells.
Essential and unexpected role of Yin Yang 1 to promote mesodermal cardiac differentiation.
Dedifferentiated fat cells convert to cardiomyocyte phenotype and repair infarcted cardiac tissue in rats.
Song IA, Oh AY, Kim JH, Choi YM, Jeon YT, Ryu JH, Hwang JW
BMC anesthesiology 2016 Feb 20;16:13
BMC anesthesiology 2016 Feb 20;16:13
Essential and unexpected role of Yin Yang 1 to promote mesodermal cardiac differentiation.
Gregoire S, Karra R, Passer D, Deutsch MA, Krane M, Feistritzer R, Sturzu A, Domian I, Saga Y, Wu SM
Circulation research 2013 Mar 15;112(6):900-10
Circulation research 2013 Mar 15;112(6):900-10
Dedifferentiated fat cells convert to cardiomyocyte phenotype and repair infarcted cardiac tissue in rats.
Jumabay M, Matsumoto T, Yokoyama S, Kano K, Kusumi Y, Masuko T, Mitsumata M, Saito S, Hirayama A, Mugishima H, Fukuda N
Journal of molecular and cellular cardiology 2009 Nov;47(5):565-75
Journal of molecular and cellular cardiology 2009 Nov;47(5):565-75
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- NKX2.5 in Human Heart. NKX2.5 was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human NKX2.5 Monoclonal Antibody (Catalog # MAB2444) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.