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Western blot
Immunoprecipitation
Other assayAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Other assay [1]
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- Product number
- A303-441A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TBC1D2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The recommended shelf life for this product is 1 year from date of receipt.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Detection of human TBC1D2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-TBC1D2 antibody A303-441A used for IP at 6 µg/mg lysate. TBC1D2 was also immunoprecipitated by rabbit anti-TBC1D2 antibodies A303-440A and A303-442A, which recognize other epitopes. For blotting immunoprecipitated TBC1D2, A303-440A was used at 0.4 µg/ml,. Detection: Chemiluminescence with an exposure time of 3 seconds.
